Background: The trans-theoretical model is a model that is very sensitive to changes in the minor progress of behavior. Therefore, the trans-theoretical model is more practical than other patterns of behavior change, which have been considered all or no to measure physical activity. This study aimed to standardize and determine the validity and reliability of the tensile movement questionnaire at the University of Medical Sciences employees. methods: In this study, after searching the texts and the study of the same questionnaires and tools and among the available and related tools, the initial questionnaire was designed. First, the validity and content of the questionnaire were measured using experts' comments. Then, the ability to understand the questionnaire based on the opinions of 10 employees was investigated. Finally, to determine the reliability with the experimental-open test method, 30 employees, Cronbach's alpha, and the correlation coefficient were obtained using SPSS software. Results: After performing the necessary reforms with experts' opinions, finally, the questionnaire with increasing the number of questions of the field of self-efficacy from 6 to 7 questions, the reduction of the number of questions of the interests of stretching movements from 10 to 5 questions, increasing the number of questions of the barriers of stretching movements from 4 to 7 questions and increasing the number of questions of In the validation stage, each 40 of the questions had acceptable content validity (8.0 to 99.0). Also, at least 80% of the questions were diagnosed in terms of the target group acceptable. Cronbach's alpha coefficient and correlation coefficient were 86.0 and 7.0, respectively. Conclusion: A designed questionnaire based on the trans-theoretical model constructs to assess educational intervention to enhance the tensile movements of employees has acceptable reliability and validity.