Background: Musculoskeletal disorders are common consequences of inappropriate computer work conditions. Employees are job groups who spend many office hours working on computers. The present study was conducted to investigate the prevalence of musculoskeletal disorders among computer users of the health network of Lordegan city and its impact on their daily activities. Methods: This research was applied in terms of purpose and was determined as one of the quantitative researches.  This descriptive-analytical study was performed with the use of systematic random sampling, and the samples were picked from the statistical population of 500 employees of Lordegan Health Network. A sample size of 217 persons was selected, which had at least one year of work experience and at least 3 hours a day working in front of a computer. The Cornell questionnaire was applied in order to specify the rate of pain and discomfort and its effect on the employees' daily activities. Then, the ROSA method was utilized to assess the ergonomics of the office strain rate of users. The results were analyzed with the use of the chi-square test and SPSS v.25 software. Results: A significant difference was seen between the rate of pain and discomfort and the effect of pain on daily activities in different body parts of computer users based on age, work experience (P<0.05). Also, there was a significant difference between ROSA final score and age variables and work experience (P<0.05). The results of the ROSA evaluation declared that 53.9% of persons were exposed at medium to high-risk levels. Conclusion: The ROSA method was appropriate for detecting risk factors for office work and was able to identify deficiencies existed in workstations. Concerning the postures and jobs in the office department, the ROSA method was able to identify musculoskeletal disorders of the office employees in health care. The CMDQ questionnaire could determine the rate and effect of pain and discomfort on different body parts for these employees.